Aberration Detection & Control in Microscopy

Supervisor: Dr Brett Patterson

Imaging in microscopy is subject to a number of sources of aberration which degrade the quality of images in terms of resolution and signal-to-noise. This is critical for fluorescence imaging in biology where the finest details reveal important information about cellular and biomolecular structure and function. This project uses a novel method to detect aberrations across the focal plane of an inverted microscope, and correct them using a deformable membrane mirror. This will correct for aberrations from the optical components of the system as well as sample aberrations due to the depth of imaging through the sample medium, and refractive index variations across the field of view.